구강편평상피암종에서 상피성장인자 수용체의 과발현과 K-ras 유전자 변이

문병출; 한세진; 정동준; 김경욱

SITE LINK

구강편평상피암종에서 상피성장인자 수용체의 과발현과 K-ras 유전자 변이
Epidermal growth factor receptor overexpression and K-ras mutation detection in the oral squamous cell carcinoma

대한구강악안면외과학회지 2011년 37권 5호 p.396 ~ 402

문병출, 한세진, 정동준, 김경욱,

소속 상세정보

문병출 ( Moon Byeong-Chool ) - 단국대학교 치과대학 구강악안면외과학교실
한세진 ( Han Se-Jin ) - 단국대학교 치과대학 구강악안면외과학교실
정동준 ( Jung Dong-Jun ) - 순천향대학교 의과대학 병리학교실
김경욱 ( Kim Kyung-Wook ) - 단국대학교 치과대학 구강악안면외과학교실

KMID : 0355620110370050396

Abstract

Introduction:Epidermal growth factor is a single-chain polypeptide consisting of 53 amino acids with potent mitogenic activity that stimulates the proliferation of a range of normal and neoplastic cells through an interaction with its specific receptor (epidermal growth factor receptor, EGFR). This interaction plays a key role in tumor progression including the induction of tumor cell proliferation. An increased EGFR copy number have been associated with a favorable response to EGFR tyrosine kinase inhibitors therapy. In contrast, K-ras mutations tend to predict a poor response to such therapy. The aim of this study was to determine the correlation between the clinicopathological factors and the up-regulation of EGFR expression and Kras mutations in oral squamous cell carcinoma.

Materials and Methods : This study examined the immunohistochemical staining of EGFR, K-ras mutation detection with peptide nucleic acid (PNA)-based real-time polymerase chain reaction (PCR) clamping in 20 specimens from 20 patients with oral squamous cell carcinoma.

Results:1. In the immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, a high level of EGFR staining was observed. The correlation between immunohistochemical EGFR expression and histological differentiation, as well as the tumor size of the specimens was significant (Pearson correlation analysis, significance [r] >0.5, P<0.05). 2. In PNA-based real-time PCR clamping analysis, a K-ras mutation was not detected in all specimens.

Conclusion:These findings suggest that the up-regulation of the EGFR may play a role in the progression and invasion of oral squamous cell carcinoma that is, independent of a K-ras mutation.